Safety of HCV-positive semen in assisted reproduction. At this appointment, the donor provides an ejaculate for evaluation only—the ejaculate is not stored and can not be used for insemination. For these reasons, it is necessary to assess the real safety of straws used for cryopreservation of human gametes and embryos, especially for routine use in assisted reproduction in conjunction with transmissible viral diseases. Additionally, if the donor donates for over one year, they must repeat the blood chemistry panel. A report from the Royal Veterinary College has shown a relationship between leakage from plastic straws used for livestock semen cryopreservation and the method used to fill the straws Russel et al. You can change or withdraw your consent at any time up to the point at which your sperm are used in treatment. This is because it has not been found to reduce the risk of infection any further.
For more than a decade, different studies have investigated HCV RNA in seminal samples, with detection levels varying according to the available methodology.
Risks for transmission of hepatitis C virus during artificial insemination.
In addition, it has been incriminated in other routes of transmission, including sexual transmission and nosocomial infections. Besides, the need for routine cryopreservation in HCV-positive male partners is questionable since it has been demonstrated by previous studies that the routine technical protocols for sperm preparation for assisted reproduction can eliminate the presence of the virus. It will be very confusing trying to work out what type of sperm to order and how much, below is a basic guide to help. Thus, to curb this risk, safety rules must be strictly respected in all assisted reproduction laboratories that include infertile couples with HCV positive men Steyaert et al. The occurrence of false-negative results due to the presence of PCR inhibitors in the ejaculates is also an important concern Semprini et al. Furthermore, a discontinuous gradient is an easy procedure to select motile sperm, which also efficiently eliminates or decreases infectious agents such as cytomegalovirus Levy et al.